This application note describes the extraction of benzodiazepine compounds from whole blood, prior to GC/MS analysis. This protocol also allows the simultaneous extraction of various other drugs of abuse classes: amphetamines, barbiturates, cocaine and opiates.
ISOLUTE® SLE+ columns with 1 mL sample capacity are used to extract whole blood samples following a straightforward sample dilution. No protein precipitation or other pre-treatment is required prior to sample loading. The sample preparation procedure delivers clean extracts, good recoveries and RSD values and LLOQs from 10 ng/mL (analyte dependant).
Tags: 2-OH-Et-Flurazepam , 7-Aminoclonazepam , 7-Aminoflunitrazepam , Alprazolam , Application Notes , Benzodiazepines , Bromazepam , Clinical , Clonazepam , Column , Diazepam , English , Estazolam , Flunitrazepam , Flurazepam , Forensic , GC-MS , Lorazepam , Midazolam , Nitrazepam , Nordiazepam , Oxazepam , Temazepam , Triazolam , Whole blood , α-OH Triazolam , α-OH alprazolam